Molecular cloning and characterization of β-mannanase B from Cellulosimicrobium sp. YB-43
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چکیده
منابع مشابه
Cloning, secretory expression and characterization of recombinant β-mannanase from Bacillus circulans NT 6.7
The mannanase gene of B. circulans NT 6.7 was cloned and expressed in an Escherichia coli expression system. The B. circulans NT 6.7 mannanase gene consists of 1,083 nucleotides encoding a 360-amino acid residue long polypeptide, belonging to glycoside hydrolase family 26. The full-length mannanase gene including its native signal sequence was cloned into the vector pET21d and expressed in E. c...
متن کاملMolecular cloning and biochemical characterization of an endo-β-mannanase gene from soybean for soybean meal improvement.
Soybean meal is the most commonly used protein source in animal feeds. Among the undesirable attributes of soybean meal is the high level of β-mannan, which was determined to be detrimental to the growth performance of animals. β-Mannan is a type of hemicellulose in the plant cell wall and can be hydrolyzed by endo-β-mannanase. The goal of this study is to isolate and characterize an endo-β-man...
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Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
متن کاملOverexpression of a Fungal β-Mannanase from Bispora sp. MEY-1 in Maize Seeds and Enzyme Characterization
BACKGROUND Mannans and heteromannans are widespread in plants cell walls and are well-known as anti-nutritional factors in animal feed. To remove these factors, it is common practice to incorporate endo-β-mannanase into feed for efficient nutrition absorption. The objective of this study was to overexpress a β-mannanase gene directly in maize, the main ingredient of animal feed, to simplify the...
متن کاملMolecular Cloning and Characterization of the Phenylalanine Aminomutase Gene From Taxus baccata L.
Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (<span style="font-variant: normal; ...
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ژورنال
عنوان ژورنال: The Korean Journal of Microbiology
سال: 2016
ISSN: 0440-2413
DOI: 10.7845/kjm.2016.6045